This quiz works best with JavaScript enabled. Home > Cbse > Class 12 > Science > Biology > Class 12 Biology Chapter 11 Biotechnology Principles And Processes – Quiz 18 🏠 Homepage 📘 Download PDF Books 📕 Premium PDF Books Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 18 (60 MCQs) Quiz Instructions Select an option to see the correct answer instantly. 1. The linking of antibodies resistance gene with plasmid vector became possible with A) DNA polymerase. B) DNA ligase. C) Exonucleases. D) Endonucleases. Show Answer Correct Answer: B) DNA ligase. 2. In biotechnology, what is the role of a plasmid in genetic engineering? A) To synthesize RNA molecules. B) To act as a vector for gene transfer. C) To degrade unwanted proteins. D) To replicate independently of chromosomal DNA. Show Answer Correct Answer: B) To act as a vector for gene transfer. 3. What is a technique that can be used to treat genetic disorders? A) Vaccine. B) Antibiotics. C) Physical therapy. D) Gene therapy. Show Answer Correct Answer: D) Gene therapy. 4. In biotechnology, which tool is used to amplify small segments of DNA for analysis? A) CRISPR-Cas9. B) Polymerase Chain Reaction (PCR). C) DNA sequencing. D) Gel electrophoresis. Show Answer Correct Answer: B) Polymerase Chain Reaction (PCR). 5. PBR322 plasmid was created by ..... A) Bolivar. B) R. Rodriguez. C) Both a and b. D) None of the above. Show Answer Correct Answer: C) Both a and b. 6. In a lab who would be the person actually running the tests and experiments on samples? A) Only the research scientist can do these things. B) Laboratory technician. C) Laboratory supervisor. D) Clinical trial coordinator. Show Answer Correct Answer: B) Laboratory technician. 7. Biotechnology has many applications. Which would not be considered a benefit of biotechnology? A) Helps to heal the world. B) Helps to fuel the world. C) Helps to travel the world. D) Helps to feed the world. Show Answer Correct Answer: C) Helps to travel the world. 8. Which of the following results would indicate that transformation of the bacteria occurred? A) Growth in LB agar. B) Growth in LB/ Amp agar by bacteria with the plasmid. C) Growth in Amp agar by bacteria without plasmid. D) None of these answers are correct. Show Answer Correct Answer: B) Growth in LB/ Amp agar by bacteria with the plasmid. 9. What type of mutation involves substituting a DNA base with a different base but still forms the same protein? A) Point missense. B) Frameshift deletion. C) Point silent. D) Frameshift insertion. E) Point nonsense. Show Answer Correct Answer: C) Point silent. 10. Given favorable conditions, which disease-causing agent could quickly multiply into colonies of billions of cells in the space of a drop of water? A) Fungi. B) Bacteria. C) Protists. D) Viruses. Show Answer Correct Answer: B) Bacteria. 11. CRISPR-Cas9 is a revolutionary tool in biotechnology. What is its main function? A) To edit genes. B) To replicate DNA. C) To synthesize proteins. D) To degrade RNA. Show Answer Correct Answer: A) To edit genes. 12. Which project is most likely to interest a scientist working at a biotechnology company? A) Study and reconstruction of fossilized bones. B) Conservation of key species within ecosystems. C) Prevention of sewage leaking into water supplies. D) Development of genetically modified corn plants. Show Answer Correct Answer: D) Development of genetically modified corn plants. 13. Humans deciding what plant or animal traits they want in a future offspring is known as ..... A) Transgenics. B) Biotechnology. C) Selective Breeding. D) Transformation. Show Answer Correct Answer: C) Selective Breeding. 14. People have been cloning plants for how long? A) Thousands of years. B) Hundreds of years. C) Only in the last ten years. D) People can't clone plants!. Show Answer Correct Answer: A) Thousands of years. 15. Which of the following examples of genetic engineering repairs existing abnormal genes? A) PCR. B) CRISPR. C) Gel Electrophoresis. D) Cloning. Show Answer Correct Answer: B) CRISPR. 16. What is the primary reason that scientists use restriction enzymes? A) To speed up the electrical current that separates DNA fragments. B) Restriction enzymes are used when the whole chromosome is too large to study easily. C) To help produce millions or billions of copies of a DNA fragment. D) All of the above. Show Answer Correct Answer: B) Restriction enzymes are used when the whole chromosome is too large to study easily. 17. Which statement is NOT an ethical concern about Genetically Modified Organisms (GMOs)? A) Farmers will be able to increase their farms and make more money. B) The long-term effects of these foods are unknown. C) A decrease in genetic diversity of plants. D) Genes for pesticide resistance might transfer to weeds by cross-pollination. Show Answer Correct Answer: A) Farmers will be able to increase their farms and make more money. 18. Why sticky ends are called so? A) Because they form covalent bonds with their complementary bases. B) Because they form ionic bonds with their complementary bases. C) Because they form peptide bonds with their complementary bases. D) Because they form hydrogen bonds with their complementary bases. Show Answer Correct Answer: D) Because they form hydrogen bonds with their complementary bases. 19. All of the following are biotechnology tools except A) Cells. B) Crystals. C) DNA. D) Protein. Show Answer Correct Answer: B) Crystals. 20. A project that analyzes the order of bases for all the genes in the human DNA. A) Human Genome Project. B) Gene Therapy. C) Cloning. D) None of the above. Show Answer Correct Answer: A) Human Genome Project. 21. To produce industrial ethanol, one needs corn, water, and what? A) Sodium and Chlorine. B) Vitamins and minerals. C) Enzymes and yeast. D) Gas and oil. Show Answer Correct Answer: C) Enzymes and yeast. 22. Gene cloning is: A) Gene is isolated and inserted in same organism. B) Gene is isolated and inserted in different organism. C) Gene is isolated and inserted in plasmid of other organism. D) Gene is isolated and inserted in chromosomal DNA. Show Answer Correct Answer: B) Gene is isolated and inserted in different organism. 23. This is the process of creating a unique image of an individual's alleles based on their size and using that image to identify an individual's DNA A) Cloning. B) Genetic Engineering. C) Gel Electrophoresis. D) DNA Fingerprinting. Show Answer Correct Answer: D) DNA Fingerprinting. 24. Which of the following could be used as a vector for a human gene? A) MRNA. B) A mitochondria. C) Bacteria. D) Bacterial Plasmid. Show Answer Correct Answer: D) Bacterial Plasmid. 25. This is the practice of choosing individuals of a species to mate based on their desirable characteristics A) Selective breeding. B) Bacterial recombination. C) Genetic modification. D) Natural selection. Show Answer Correct Answer: A) Selective breeding. 26. The correct order of steps in Polymerase Chain Reaction (PCR) is ..... A) Denaturation, Extension, Annealing. B) Denaturation, Annealing, Extension. C) Annealing, Extension, Denaturation. D) Extension, Denaturation, Annealing. Show Answer Correct Answer: B) Denaturation, Annealing, Extension. 27. 1) What would be a positive reason to genetically alter Fraser Fir trees in North Carolina to make them more resistant to rot? A) By producing crops that are frost resistant. B) By making robots to replace large farm machines. C) The population of fir trees would increase and grow healthier. D) By increasing the use of wind farms that produce electricity. Show Answer Correct Answer: C) The population of fir trees would increase and grow healthier. 28. Restriction enzymes ..... A) Cut DNA at a specific site. B) Can be used to isolate a gene of interest. C) Can be used to cut vector DNA. D) Do all of these things. Show Answer Correct Answer: D) Do all of these things. 29. Human insulin is being commercially produced from a transgenic species of A) Rhizobium. B) Eschericha. C) Saccharomyces. D) Mycobacterium. Show Answer Correct Answer: B) Eschericha. 30. What is the basic definition of biotechnology? A) Study of human genetics. B) Study of plant diseases. C) Use of living organisms to make products. D) Learning about plant parts. Show Answer Correct Answer: C) Use of living organisms to make products. 31. Nata de coco adalah salah satu hasil olahan yang memanfaatkan mikroorganisme A) Neurospora crassa. B) Eschericia coli. C) Acetobacter xylinum. D) Sacharomyces. Show Answer Correct Answer: C) Acetobacter xylinum. 32. The scientist that works in a laboratory to study the effects new compounds interact with disease causing organisms to develop new drugs. A) Pharmaceutical scientist. B) Biochemist. C) Microbiologist. D) Chemist. Show Answer Correct Answer: A) Pharmaceutical scientist. 33. Which of the following would be an example of gene therapy technology? A) Cutting DNA into fragments with restriction enzymes. B) Modifying E. coli to produce indigo dye for coloring denim blue jeans. C) Development of a nasal spray that contains copies of the normal gene that is defective in person with cystic fibrosis. D) Separation DNA fragments using gel electrophoresis. Show Answer Correct Answer: C) Development of a nasal spray that contains copies of the normal gene that is defective in person with cystic fibrosis. 34. What must be isolated from histone proteins in the DNA extraction process? A) DNA. B) Organelles. C) Chromosomes. D) Nucleus. Show Answer Correct Answer: A) DNA. 35. What does a cell biologists study? A) Structure of cells. B) Functions of cells. C) Types of cells. D) All of the these. Show Answer Correct Answer: D) All of the these. 36. Identifying a freeze resistant gene in a fish and placing it into a tomato plant is an example of A) Transgenic organisms. B) Inbreeding. C) Genome. D) Hybridization. Show Answer Correct Answer: A) Transgenic organisms. 37. The purpose gene therapy in treating disorders, such as cystic fibrosis and SCIDS, is to A) Replace faulty genes with normal ones. B) Determine the sequence of genes. C) Remove mutations from genes. D) Change dominant alleles to recessive alleles. Show Answer Correct Answer: A) Replace faulty genes with normal ones. 38. The latter R in EcoRI is derived from A) The name of genus. B) The name of strain. C) The name of species. D) The term restriction. Show Answer Correct Answer: B) The name of strain. 39. For a DNA to function as a cloning vector the most essential requirement is- A) Several selectable markers. B) Circular nature. C) 'ori' sequence. D) Multiple restriction sites. Show Answer Correct Answer: C) 'ori' sequence. 40. Dampak negatif yang ditimbulkan dari pemanfaatan biotehnologi trangenik dalam pelestarian spesies tertentu adalah ..... A) Munculnya bibit unggul. B) Cepat bereproduksi. C) Timbunya jenis baru. D) Kerusakan plasma nutfah. Show Answer Correct Answer: D) Kerusakan plasma nutfah. 41. Golden rice is genetically engineered to produce ..... A) Vitamin C. B) Lactase. C) Insulin. D) $\beta$-carotene. Show Answer Correct Answer: D) $\beta$-carotene. 42. Stirred-tank bioreactors have been designed for A) Ensuring anaerobic conditions in the culture vessel. B) Availability of oxygen throughout the process. C) Addition of preservatives to the product5%. D) Purification of the product. Show Answer Correct Answer: B) Availability of oxygen throughout the process. 43. Mikroorganisme yang digunakan dalam proses pembuatan Natta de Coco adalah ..... A) Saccharomyces Cerevisceae. B) Acetobacter Xylinum. C) Lactobacillus casei. D) Aspergillus wentii. Show Answer Correct Answer: B) Acetobacter Xylinum. 44. Who is responsible for running the clinical trials? A) Biochemist. B) Clinical trials monitor. C) Data specialists. D) Clinical trials research associate. Show Answer Correct Answer: D) Clinical trials research associate. 45. Name the first restriction enzyme discovered? A) Hind II. B) BamHI. C) Hind III. D) EcoRI. Show Answer Correct Answer: A) Hind II. 46. Multiple Choice Questions (1-25)-Competent cells are prepared using: A) Sodium chloride. B) Calcium chloride. C) Potassium iodide. D) Ammonium sulphate. Show Answer Correct Answer: B) Calcium chloride. 47. A:ADA deficiency is a genetic disorder. R:It is caused due to defective adenosine deaminase enzyme. Choose the correct option about A and R. A) Both A and R are true, and R is the correct explanation of A. B) Both A and R are true, but R is not the correct explanation of A. C) A is true, but R is false. D) A is false, but R is true. Show Answer Correct Answer: A) Both A and R are true, and R is the correct explanation of A. 48. Find the odd one wrt to PCR A) Renaturation at 50-60 degrees. B) Extension at 72 degrees. C) Denaturation at 94 degrees. D) Denaturation at 72 degrees. Show Answer Correct Answer: B) Extension at 72 degrees. 49. Which of the following tools is commonly used in biotechnology for cutting DNA at specific sequences? A) Restriction Enzymes. B) DNA Ligase. C) RNA Polymerase. D) DNA Polymerase. Show Answer Correct Answer: A) Restriction Enzymes. 50. Which is an example of selective breeding? A) Asexual reproduction. B) Survival of the fittest. C) Dog breeding for desired characteristics. D) Natural selection. Show Answer Correct Answer: C) Dog breeding for desired characteristics. 51. Construction of a recombinant DNA involves A) Cleaving and rejoining DNA SEGMENT with ligase alone. B) Cleaving DNA segment with ligase and joining them with endonuclease. C) Cleaving DNA segment with endonuclease and joining them with ligase. D) Cleaving and rejoining DNA segment with endonuclease alone. Show Answer Correct Answer: C) Cleaving DNA segment with endonuclease and joining them with ligase. 52. Which of the below biotechnological advances is MOST beneficial to Florida FARMERS? A) Development of disease resistant plants for higher yields. B) The ability to create more antibiotics from bacteria. C) Producing enzymes to eliminate pollutants. D) Development of more effective human vaccines. Show Answer Correct Answer: A) Development of disease resistant plants for higher yields. 53. Genetic modification is a technique used in A) Herbicide-resistant crops. B) Transgenic organisms. C) Cloning. D) All of the above. Show Answer Correct Answer: D) All of the above. 54. What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?I) Grow transgenic bacteria.II) Cut the plasmid DNA using restriction enzymes.III) Extract plasmid DNA from a bacterial cell.IV) Create recombinant DNA.V) Combine the gene of interest to the plasmid, creating recombinant DNA A) I, II, IV, III, V. B) III, IV, V, I, II. C) II, III, V, IV, I. D) III, II, IV, V, I. Show Answer Correct Answer: D) III, II, IV, V, I. 55. What enzyme is used to cut a specific spot in the plasmid? A) Helicase. B) Restriction enzyme. C) Ligase. D) Polymerase. Show Answer Correct Answer: B) Restriction enzyme. 56. What is tissue culture in plant biotechnology? A) Studying plant tissues under a microscope. B) Eating plant tissues. C) Growing plants in a laboratory from tissues. D) Drawing diagrams of plant tissues. Show Answer Correct Answer: C) Growing plants in a laboratory from tissues. 57. Disorder in which $\beta$-lymphocytes and T-lymphocytes are not formed is ..... A) SCID. B) AIDS. C) Muscular Dystrophy. D) Cystic Fibrosis. Show Answer Correct Answer: A) SCID. 58. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes? A) Sludge digester. B) Industrial oven. C) BOD Reactor. D) Bioreactor. Show Answer Correct Answer: D) Bioreactor. 59. What is a fermented alcohol made from the sugar found in corn? A) Hydrogen. B) Ethanol. C) Acetone. D) Oxygen. Show Answer Correct Answer: B) Ethanol. 60. Plasmids are found in bacteria. Scientists use plasmids to A) Insert eukaryotic genes into bacteria. B) Insert eukaryotic genes into humans. C) Insert clones into the parent organism. D) None of the above. Show Answer Correct Answer: A) Insert eukaryotic genes into bacteria. ← PreviousNext →Related QuizzesScience QuizzesClass 12 QuizzesClass 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 1Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 2Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 3Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 4Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 5Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 6Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 7Class 12 Biology Chapter 11 Biotechnology Principles And Processes Quiz 8 🏠 Back to Homepage 📘 Download PDF Books 📕 Premium PDF Books